METHODS OF INTEGRATED MICROFLUIDIC PROCESSING FOR PREVENTING SAMPLE LOSS

The present disclosure provides methods of pre-loading processing and loading of an aqueous sample solution to a device such as a microfluidic device or chip in order to prevent sample loss due to dead volume retention of the aqueous sample solution or sample sticking to wall of test tubes or reaction containers, methods of integrated processing of an aqueous sample solution on an on-chip well and direct feeding of the processed in-well solution into microfluidic circuits for further processing without any transfer apparatus in order to prevent sample loss, methods of utilizing phase separation of different matters for reactions with any number of steps of reagent addition at any desired volume or mass ratios determined by the chosen reaction and transfer apparatus while preventing loss of rare or valuable samples, methods of fabricating a portable all-in-one device and using it for applications including isothermal reactions, and methods of using a portable and integrated device at a remote site for medical doctors, scientists, police officers, and the like to perform on-site studies.